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Enhanced PDMS Functionalization for Organ-on-a-Chip Platforms Using Ozone and Sulfo-SANPAH: A Simple Approach for

Mitzi Pérez-Calixto1, Cindy Peto-Gutiérrez1, Alyssa Shapiro1

  • 1Facultad de Ciencias, Universidad Nacional Autónoma de México, Circuito Exterior S/N, Ciudad Universitaria, Ciudad de México, 04510, Mexico.

Advanced Healthcare Materials
|April 2, 2025
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Summary

A new one-step surface modification for polydimethylsiloxane (PDMS) using ozone and sulfo-SANPAH (SS) creates a stable hydrophilic surface for organ-on-a-chip (OOC) systems, improving cell culture. This method enhances cell adhesion and network formation in microfluidic devices.

Keywords:
LSECfunctionalizationhepatocytesorgan‐on‐a‐chippolydimethylsiloxanesulfo‐SANPAH

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Area of Science:

  • Biomaterials Science
  • Microfluidics
  • Cell Biology

Background:

  • Surface modification of polydimethylsiloxane (PDMS) is crucial for organ-on-a-chip (OOC) applications.
  • Conventional UV-based surface modifications are temporary and limit long-term cell culture success.

Purpose of the Study:

  • To develop a simple, stable, and effective one-step surface modification method for PDMS in OOC systems.
  • To enhance the biocompatibility and functionality of PDMS microchannels for cell-based assays.

Main Methods:

  • Physicochemical surface modification of PDMS microchannels using ozone and sulfo-SANPAH (SS).
  • Characterization using spectroscopy, microscopy, and contact angle measurements.
  • Culturing human liver sinusoidal endothelial cells (LSEC) and murine primary hepatocytes in modified OOC channels for up to 7 days.

Main Results:

  • Successful transition of PDMS surface from hydrophobic to hydrophilic (contact angle reduced to 20.8° after ECM deposition).
  • Demonstrated strong cell adhesion of LSECs under laminar flow.
  • Formation of a well-established canaliculi network by hepatocytes in OOC channels.

Conclusions:

  • The one-step ozone and SS modification provides a simple and robust method for PDMS surface functionalization in OOC platforms.
  • This approach is suitable for beginners and enhances cell adhesion and function, paving the way for advanced microfluidic cell culture applications.