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Barcode gap delimitation and phylogenetic analyses in integrative earthworm taxonomy: Dendrobaena byblica complex
Robabeh Latif1, Atabak Roohi Aminjan2, Masoumeh Malek3
1Farzanegan Campus; Semnan University; Semnan; Iran. r.latif@semnan.ac.ir.
Abstract:
Dendrobaena byblica (Rosa, 1893) is a species complex consisting of at least 17 nominal taxa and with Circum-Mediterranean distribution. The taxonomic classification of this group based on shape of internal structures and external characteristics indicated that this species complex indeed consists of several clades. More molecular studies are needed to confirm this issue. In this study, samples from the Hyrcanian forests in the north of Iran were studied, which were genetically different from the sequences retrieved from GenBank. The results of the ABGD analyses suggest that the genetic distance for species delimitation in D. byblica is 9%, a much lower value than that reported for other species included in the family Lumbricidae. The range of COI K2p genetic distance between GenBank sequences of Dendrobaena and examined species in the present study, is 12.3-24.6%. Assessing whether these genetic variations correspond to different biological species or correspond to intraspecific phenotypic variability will require further studies using other genetic molecular markers.
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