Embracing the unknown: Proteomic insights into the human microbiome
Shuqin Zeng1, Alexandre Almeida2, Dezhi Mu1
1Department of Pediatrics, West China Second University Hospital, Sichuan University, Chengdu 610041, China; Key Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of Education, West China Second University Hospital, Sichuan University, Chengdu 610041, China.
Cell Metabolism
|April 2, 2025
Summary
Investigating microbiome proteins reveals active microbial functions and host interactions. This approach quantifies the dietary exposome and identifies health and disease proteomic signatures.
Area of Science:
- Microbiology
- Proteomics
- Systems biology
Background:
- Human microbiome research increasingly focuses on protein-level functions.
- Understanding microbial roles in host health and disease requires functional insights.
Purpose of the Study:
- To introduce and demonstrate a metagenome-informed metaproteomics approach.
- To functionally explore species-level microbiome-host interactions.
- To quantify the dietary exposome and identify disease-specific proteomic signatures.
Main Methods:
- Metagenome-informed metaproteomics.
- Application in mouse and human studies.
- Proteomic signature analysis.
Main Results:
- Demonstrated potential for functional microbiome exploration.
- Successful quantification of the dietary exposome.
- Identification of proteomic signatures associated with health and inflammatory bowel disease.
Conclusions:
- Metagenome-informed metaproteomics is a powerful tool for microbiome functional analysis.
- This approach can elucidate microbiome-host interactions and the dietary exposome.
- It holds promise for identifying biomarkers in health and disease states.
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