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Updated: May 16, 2025

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
Published on: August 23, 2022
Semi-automated image acquisition and analyses for broad users utilizing macro keyboards
Takaaki Watanabe1, Toshiyasu Taniguchi1
1Department of Molecular Life Science, Tokai University School of Medicine, 143 Shimokasuya, Isehara, Kanagawa 259-1193, Japan.
Abstract:
Scientific research relies on microscopy. However, manual image acquisition and analysis are inefficient and susceptible to errors. Fully automated workflows are often task-specific, and current AI-based systems are costly and may face difficulties in new scenarios. Here, we introduce a semi-automated system utilizing macro keyboards to streamline workflows. Programming multi-action keys for tasks such as focusing, image capture and data analysis reduces the manual input, boosting efficiency and accuracy. This intuitive system saves time for both experienced users and trainees. This cost-effective solution improves accessibility, flexibility and usability, supporting not only diverse imaging applications but also broader scientific instrumentation processes.

