Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Protein Dynamics in Living Cells

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

From Sea to Cell: <i>Ascophyllum nodosum</i> and <i>Fucus vesiculosus</i> Extracts Attenuate NF-κB-Mediated Inflammation and Protect Intestinal Barrier Integrity-A Comprehensive Analysis Applying In Vitro and In Vivo Models.

Marine drugs·2026

Same author

Myosin II regulates cellular thermo-adaptability and the efficiency of immune responses.

Developmental cell·2025

Same author

Cell aging - a relevant factor in live cell microscopy (mini-review).

Progress in biophysics and molecular biology·2025

Same author

Homeostatic Counter-Regulation Mediates Spermidine-Induced Triacylglyceride Reduction in Drosophila melanogaster-From Phenotype to Molecular Mechanism.

FASEB journal : official publication of the Federation of American Societies for Experimental Biology·2025

Same author

Antioxidant Power of Brown Algae: <i>Ascophyllum nodosum</i> and <i>Fucus vesiculosus</i> Extracts Mitigate Oxidative Stress In Vitro and In Vivo.

Marine drugs·2025

Same author

Allium ursinum leaves aqueous extract alleviates Pseudomonas aeruginosa infection in Caenorhabditis elegans.

Phytomedicine : international journal of phytotherapy and phytopharmacology·2025

Search research articles

Related Experiment Video

Updated: May 16, 2025

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 19, 2010

Soft lithography-based biomolecule patterning techniques and their applications in subcellular protein interaction

Tina Karimian¹, Peter Lanzerstorfer¹, Julian Weghuber^1,2

¹University of Applied Sciences Upper Austria, Center of Excellence Food Technology and Nutrition, Stelzhamerstrasse 23, 4600, Wels, Austria.

Materials Today. Bio

|April 3, 2025

Summary

Soft lithography techniques enable precise micro- and nanoscale biomolecule patterning for subcellular protein-protein interaction (PPI) analysis. This review details advances, challenges, and future applications in this rapidly evolving field.

Keywords:

Protein patterning Protein-protein-interactions Soft lithography contact printing Subcellular micropatterning

More Related Videos

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Published on: September 6, 2011

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

Published on: October 11, 2019

Related Experiment Videos

Last Updated: May 16, 2025

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

In-vivo Detection of Protein-protein Interactions on Micro-patterned Surfaces

Published on: March 19, 2010

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Creating Two-Dimensional Patterned Substrates for Protein and Cell Confinement

Published on: September 6, 2011

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

Light-Induced Molecular Adsorption of Proteins Using the PRIMO System for Micro-Patterning to Study Cell Responses to Extracellular Matrix Proteins

Published on: October 11, 2019

Area of Science:

Biotechnology
Nanotechnology
Molecular Biology

Background:

Soft lithography offers versatile methods for creating micro- and nanoscale biomolecule features on solid substrates.
Biomolecule patterning is crucial for understanding cellular functions and interactions.

Purpose of the Study:

To review advances in soft lithography for biomolecule deposition.
To highlight applications in subcellular protein-protein interaction (PPI) analysis.

Main Methods:

Micro-contact printing (μCP)
Nano-contact printing (nCP)
Capillary nanostamping
Polymer-pen-lithography (PPL)

Main Results:

Soft lithography techniques are effective for patterning biomolecules on diverse substrates.
Challenges in substrate selection, surface activation, and stamp development are addressed.
Specific advantages, limitations, and solutions for printing various biomolecules are discussed.

Conclusions:

Soft lithography-based biomolecule patterning is vital for subcellular PPI analysis.
These techniques integrate complex cellular events into PPI readout modalities.
Future technologies promise enhanced applications in biomolecular patterning.