Leveraging dielectrophoresis in inertial flow for versatile manipulation of micro and nanoparticles

The manipulation of micro and nanoparticles has extensive applications in biomedical research, clinical diagnostics, environmental monitoring, drug discovery, and the mining industry. Dielectrophoresis (DEP) utilises nonuniform electric fields to manipulate particles, offering a label-free, high-precision, and non-invasive method for both natural and synthetic particles. DEP manipulation has been well studied in the Stokes flow region with ultra-low Reynolds numbers (Re ≪ 1), where viscous effects dominate. However, its application in the inertial flow regime remains largely unexplored. This study aims to bridge the gap by coupling DEP and inertial flow for the manipulation of particles across micro and nano scales. First, we theoretically analysed the physical coupling of DEP and inertial lift forces along the vertical direction in microchannels, utilising symmetrical interdigitated electrode (IDE) arrays patterned on the top and bottom channel surfaces. We then experimentally investigated how the vertical coupling of DEP and inertial lift forces affects particle lateral focusing properties. The effects of DEP along the vertical direction were leveraged and amplified by the inertial effects along the lateral direction. Finally, we applied DEP in the inertial flow regime for size-based and dielectric property-based separation of particles and cells, as well as nanoparticle focusing and filtration. We believe that leveraging DEP in inertial flow will advance the field by providing a versatile and powerful method for the manipulation of micro and nanoparticles.