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Updated: May 17, 2025

Substrate Generation for Endonucleases of CRISPR/Cas Systems
Published on: September 8, 2012
AcrIE7 inhibits the CRISPR-Cas system by directly binding to the R-loop single-stranded DNA
Do Yeon Kim1, So Yeon Lee1, Hyun Ji Ha1
1Department of Global Innovative Drugs, College of Pharmacy, Chung-Ang University, Seoul 06974, Republic of Korea.
Abstract:
The CRISPR-Cas system is a well-known adaptive immune system in bacteria, and a prominent mechanism for evading this immunity involves anti-CRISPR (Acr) proteins, which employ various methods to neutralize the CRISPR-Cas system. In this study, using structural and biochemical analyses, we revealed that AcrIE7 binds to the single-stranded DNA in the R-loop formed when Cascade encounters the target DNA, thereby preventing Cas3 from cleaving the DNA. This represents a different inhibition strategy distinct from previously reported Acr mechanisms and offers insights into CRISPR-Cas inhibition.
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