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Updated: May 16, 2025

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

Published on: February 28, 2015

Theoretical Basis for the Highly Efficient Aptamer Selection Using Unique Molecular Identifiers.

Zhenhao Long¹, Jingjing Yu¹, Tao Bing^1,2,3,4

¹Zhejiang Cancer Hospital, The Key Laboratory of Zhejiang Province for Aptamers and Theranostics, Hangzhou Institute of Medicine (HIM), Chinese Academy of Sciences, 310022 Hangzhou, Zhejiang, China.

Analytical Chemistry

|April 3, 2025

Summary

This summary is machine-generated.

This study theoretically analyzes a novel aptamer selection strategy using unique molecular identifiers (UMIs) for efficient, single-round aptamer discovery. The method reduces bias and nonspecific binding, enhancing high-affinity aptamer isolation.

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Last Updated: May 16, 2025

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay

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Published on: February 28, 2015

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Primer-Free Aptamer Selection Using A Random DNA Library

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Published on: July 26, 2010

Area of Science:

Biotechnology
Molecular Biology
Genomics

Background:

Traditional aptamer selection methods often involve multiple rounds, leading to PCR bias and sequence overenrichment.
Developing rapid and efficient aptamer discovery techniques is critical for diverse applications.

Purpose of the Study:

To conduct a systematic theoretical analysis of a previously reported single-round aptamer selection strategy.
To elucidate the theoretical basis, advantages, and applicability of this novel method.

Main Methods:

Theoretical analysis of an aptamer selection strategy integrating unique molecular identifiers (UMIs) and DNA barcoding.
High-throughput sequencing for accurate quantification of aptamer candidates.
Investigating the feasibility of selecting aptamers from low-enriched DNA libraries.

Main Results:

The strategy effectively mitigates PCR bias and sequence overenrichment inherent in traditional methods.
Theoretical analysis confirms the strategy's effectiveness in reducing nonspecific binding.
Demonstrates increased success in selecting high-affinity aptamers.

Conclusions:

The theoretical analysis supports the broad applicability of the single-round aptamer selection strategy.
This method offers a pathway for widespread adoption in high-efficiency aptamer discovery.
Facilitates advancements in aptamer-based cell atlas development.