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Updated: May 21, 2025

Culture of Embryonic Mouse Cochlear Explants and Gene Transfer by Electroporation
Published on: January 12, 2015
Ectoderm barcoding reveals neural and cochlear compartmentalization
Sandra de Haan1,2,3, Jingyan He1, Agustin A Corbat1
1Department of Cell and Molecular Biology, Karolinska Institutet, Solna, Sweden.
None:
Placodes and the neural crest are defining features of vertebrates. In this study, we investigate their lineages in mice using in utero approaches. We demonstrated that nanoinjection at embryonic day 7.5 targeted the ectoderm, including the future nervous system, placodes, and neural crest, allowing highly efficient manipulation of the future nervous system and inner ear. By using heritable DNA barcodes and high-throughput next-generation single-cell lineage tracing, we elucidated convergent differentiation pathways and identified distinct nervous system-, neural crest-, and otic placode-derived lineages. Clonal analyses identified early neural and cochlear compartmentalization, linking differentiated cell types to their progenitors or cellular siblings. This provides foundational insights for neuroscience and developmental biology.
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