Related Experiment Video
Updated: May 16, 2025

Generation and Assembly of Virus-Specific Nucleocapsids of the Respiratory Syncytial Virus
Published on: July 27, 2021
Structure of the turnip yellows virus particles
Joséphine Lai-Kee-Him1, Stefano Trapani1, Sylvaine Boissinot2
1Centre de Biologie Structurale (CBS), Univ Montpellier, CNRS, INSERM, Montpellier, France.
Abstract:
Turnip yellows virus (TuYV) is a plant virus infecting important crops such as oilseed rape. TuYV is phloem-restricted and transmitted by aphids. The capsid contains two subunit types: the major capsid protein (CP) and a minor component (RTP∗) which arises from the C-terminal cleavage of a readthrough product (RTP). RTP∗ contains the CP sequence fused with a structured domain, denoted NRTD, which is a key determinant of virus transmission. Though both CP and RTP∗ are involved in virus movement and aphid transmission, how RTP∗ is incorporated into the capsid is poorly understood. We present here the structural characterisation, by immunogold labelling and 3D cryo-EM, of the wild-type TuYV and a mutant whose capsid contains the CP only. We show that incorporation of RTP∗ does not impair the capsid structure, and the NRTD does not adopt well-defined positions at the capsid surface. The number of incorporated RTP∗s suggests a random insertion.
More Related Videos
08:14Analysis of the Solvent Accessibility of Cysteine Residues on Maize rayado fino virus Virus-like Particles Produced in Nicotiana benthamiana Plants and Cross-linking of Peptides to VLPs
Published on: February 14, 2013
09:27Methodology for the Efficient Generation of Fluorescently Tagged Vaccinia Virus Proteins
Published on: January 17, 2014
Related Concept Videos
Viral Structure
What are Viruses?
Intracellular Movement of Viruses and Bacteria
Protein Complex Assembly
Many viruses self-assemble into a fully functional unit using the infected host cell to...
Leaky Scanning
Retrovirus Life Cycles