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DNA Microarrays

DNA Microarrays

Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...

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Related Experiment Video

Updated: Jul 4, 2026

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

Published on: March 5, 2017

Comparative transcriptome analysis identified candidate genes associated with kernel row number in maize.

Shukai Wang¹, Yancui Wang¹, Xitong Xu¹

¹College of Agronomy, Shandong Agricultural University, Taian, Shandong, China.

|April 4, 2025

Summary

This summary is machine-generated.

This study identified 11,897 differentially expressed genes in maize kernel row number development. Key candidate genes involved in development and hormone signaling were pinpointed for future research.

Keywords:

Comparative transcriptome Kernel row number Maize Quantitative trait locus

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Last Updated: Jul 4, 2026

Experimental Design for Laser Microdissection RNA-Seq: Lessons from an Analysis of Maize Leaf Development

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Published on: March 5, 2017

Discrimintion and Mapping of the Primary and Processed Transcripts in Maize Mitochondrion Using a Circular RT-PCR-based Strategy

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Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling

Published on: May 21, 2020

Area of Science:

Plant Genetics
Maize Breeding
Molecular Biology

Background:

Kernel row number (KRN) is a vital quantitative trait in maize influencing crop yield.
Understanding the genetic basis of KRN is crucial for maize improvement.
Limited genes controlling KRN have been identified to date.

Purpose of the Study:

To identify candidate genes controlling kernel row number (KRN) in maize.
To analyze transcriptome-wide gene expression during early ear development in maize.
To provide genetic resources for elucidating KRN molecular mechanisms.

Main Methods:

Transcriptome analysis of two contrasting maize inbred lines at five developmental stages.
Differential gene expression analysis to identify line-specific DEGs.
Gene ontology enrichment and QTL/QTN analysis to identify candidate genes.

Main Results:

11,897 line-specific differentially expressed genes (DEGs) were detected across five developmental stages.
Gene expression patterns were divided into two phases (V6-V8 and V9-V10) with distinct enriched pathways.
92 high-probability candidate genes, including transcription factors and hormone-related genes, were identified within QTLs/QTNs.

Conclusions:

The study identified key candidate genes regulating maize kernel row number development.
Transcriptional pathways related to morphogenesis, differentiation, and hormone regulation are crucial in early ear development.
Provides valuable genetic resources for future gene cloning and maize breeding efforts.