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Updated: May 16, 2025

Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism
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Protocol for the Solid-phase Synthesis of Oligomers of RNA Containing a 2'-O-thiophenylmethyl Modification and Characterization via Circular Dichroism

Published on: July 28, 2017

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Homocysteine Thiolactone Modification of Ribonuclease A: Thermodynamics and Kinetics

Kabira Sabnam1, Swagata Dasgupta1

  • 1Department of Chemistry, Indian Institute of Technology Kharagpur, Kharagpur, India.

Proteins
|April 4, 2025
PubMed
Summary

Homocysteine thiolactone modifies proteins like Ribonuclease A (RNase A), forming dimers and reducing enzyme activity. This study reveals structural changes and functional impairment in modified RNase A, offering insights into disease mechanisms.

Keywords:
dimer formationkinetic parametersribonuclease Aribonucleolytic activitythermodynamic parameters

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