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Clathrin is required for DNA damage repair 
Tongbin Yang1,2,3,4, Xuerui Lu1,2,3,4, Leilei Duan1,2,3,4
1Hubei Hongshan Laboratory, Huazhong Agricultural University, Wuhan, 430070, China.
Journal of Integrative Plant Biology
|April 4, 2025
Summary
Clathrin, a protein known for cell uptake, is essential for DNA repair through homologous recombination in Arabidopsis. CLATHRIN LIGHT CHAIN 2
Area of Science:
- Molecular Biology
- Genetics
- Cell Biology
Background:
- Clathrin is a protein complex primarily known for its role in endocytosis.
- Homologous recombination is a critical DNA repair pathway for double-strand breaks.
Purpose of the Study:
- To investigate the role of clathrin in DNA repair mechanisms.
- To explore potential non-canonical functions of clathrin in the nucleus.
Main Methods:
- Genetic screening in the model plant Arabidopsis thaliana.
- Analysis of clathrin's role in homologous recombination.
- Subcellular localization studies of CLATHRIN LIGHT CHAIN 2.
Main Results:
- Genetic screening identified clathrin as necessary for homologous recombination in Arabidopsis.
- CLATHRIN LIGHT CHAIN 2 was observed to localize within the nucleus.
- This suggests a novel nuclear function for clathrin.
Conclusions:
- Clathrin plays a previously unrecognized role in nuclear DNA repair via homologous recombination.
- The nuclear localization of CLATHRIN LIGHT CHAIN 2 supports non-canonical functions of clathrin beyond endocytosis.
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