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Related Concept Videos

Bioreactor Design and Operational System01:29

Bioreactor Design and Operational System

Bioreactors are engineered vessels designed to cultivate microorganisms under controlled conditions for industrial bioprocessing. They maintain sterility and allow precise regulation of pH, temperature, oxygen, and nutrient levels to optimize microbial growth and metabolite production. Bioreactors range from small laboratory units of 1 liter to industrial systems holding up to 500,000 liters, though only about 75% of their volume is actively used for fermentation. The remaining headspace...
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Maintaining optimal conditions within fermenters is essential for maximizing microbial productivity and ensuring process efficiency. This lesson focuses on key parameters—temperature, foam, pH, carbon dioxide, oxygen, and pressure—and their precise measurement and control strategies in fermentation systems.Temperature ControlTemperature regulation is critical due to the exothermic nature of many fermentation processes. In small laboratory fermenters, temperature is commonly monitored using...
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In aerobic fermentations, oxygen is vital for microbial growth and metabolite production. Since air comprises only about 20% oxygen and the gas is poorly soluble in water—just 9 ppm at 20°C—supplying sufficient oxygen becomes a critical challenge, especially in high-demand processes like yeast growth or citric acid production. Even a fully saturated broth may offer only a few seconds of oxygen availability.To address this, sterile or scrubbed air is introduced into the fermentor via a sparger...

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Microalgae Cultivation and Biomass Quantification in a Bench-Scale Photobioreactor with Corrosive Flue Gases
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A Tool for On-Line Monitoring Microalgal Bioprocesses Based on Gas Balance Analysis.

Guillaume Cogne1, Fernando Ferrel Ballestas1,2, Mariana Titica1

  • 1Nantes Université, Oniris, CNRS, GEPEA, UMR 6144, Saint-Nazaire, France.

Biotechnology and Bioengineering
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Summary

This study presents a new method to monitor microalgae growth and mass transfer in photobioreactors. It accurately predicts biomass and carbon dynamics, optimizing photobioreactor performance.

Keywords:
biomass estimationelemental balancesmass transfer coefficientsmicroalgae cultivationoxygen production ratephotobioreactor

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Area of Science:

  • Biotechnology
  • Bioprocess Engineering
  • Algal Biotechnology

Background:

  • Photobioreactors are crucial for microalgae cultivation.
  • Accurate monitoring of microalgae growth and mass transfer is essential for process optimization.
  • Current methods may be limited in controlled, non-limiting conditions.

Purpose of the Study:

  • To develop and validate a novel indirect monitoring method for microalgae growth.
  • To evaluate the mass transfer efficiency in photobioreactors.
  • To provide a tool for enhanced process control and optimization.

Main Methods:

  • Utilized elemental composition, gas transfer rates, and oxygen production data.
  • Employed indirect measurements to estimate biomass concentration, total inorganic carbon, and nitrogen.
  • Calculated gas-liquid mass transfer coefficients.

Main Results:

  • Accurately predicted microalgae biomass growth.
  • Successfully characterized carbon dynamics within the photobioreactor.
  • Effectively determined gas-liquid mass transfer coefficients.
  • Demonstrated the method's robustness under controlled conditions.

Conclusions:

  • The novel method provides accurate insights into microalgae growth and carbon dynamics.
  • It enables effective characterization of mass transfer coefficients in photobioreactors.
  • This approach offers a valuable tool for optimizing photobioreactor performance and control.