Diploid chromosome-level genome assembly and annotation for Lycorma delicatula

Area of Science:

• Genomics
• Invasive Species Biology
• Agricultural Entomology

Background:

• The spotted lanternfly (Lycorma delicatula) is an invasive planthopper threatening agriculture globally.
• Effective management strategies and understanding invasion biology necessitate robust genomic resources.

Purpose of the Study:

• To generate a high-quality, chromosome-level genome assembly for Lycorma delicatula.
• To provide genomic resources for pest management and invasive species research.

Main Methods:

• PacBio long-read sequencing and Hi-C technology for genome assembly.
• RNA-sequencing for gene annotation.
• Whole genome resequencing for sex chromosome identification.

Main Results:

• A 2.2 Gbp, 13-chromosome genome assembly was produced.
• Over 12,000 protein-coding genes were identified and annotated.
• Chromosome 4 was identified as the sex chromosome, indicating an XO sex-determination system.

Conclusions:

• The reference-quality genome assembly is a vital resource for developing and optimizing spotted lanternfly control methods.
• This genomic resource will advance the study of invasive species genomics.