Isolation, Screening, and Identification of Staphylococcus epidermidis with Effective Probiotic Attributes

Area of Science:

• Microbiology
• Biotechnology
• Pharmacology

Background:

• Staphylococcus epidermidis is a common bacterium with potential therapeutic applications.
• Investigating novel strains for probiotic, antioxidant, and anti-cancer properties is crucial for developing new treatments.

Purpose of the Study:

• To evaluate the probiotic, antioxidant, antimicrobial, and anti-cancer potential of the Staphylococcus epidermidis SAS1 strain.
• To assess the safety of the SAS1 strain for human use.

Main Methods:

• Probiotic potential assessed via in-vitro gastric condition tolerance (acidic pH, bile salts).
• Antioxidant activity evaluated through free radical scavenging and reducing power assays.
• Anti-cancer activity determined using MTT assay on HT-29 colorectal adenocarcinoma cells.

Main Results:

• The SAS1 strain demonstrated safety with no gelatinase, DNase, or hemolytic activity.
• Significant free radical scavenging and reducing antioxidant power were observed.
• The strain exhibited potent anti-cancer effects against HT-29 cells with an IC50 of 8.30 µg/mL, inducing apoptosis.
• Lipase inhibition activity was also noted.

Conclusions:

• Staphylococcus epidermidis SAS1 strain possesses significant in vitro probiotic, antioxidant, antimicrobial, and anti-cancer properties.
• The strain's safety profile and anti-cancer efficacy suggest potential for therapeutic applications, including colon cancer treatment.