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Lymphangiogenesis in mouse embryonic and early postnatal ovaries
Hongyu Su1, Zhilin Zhou2, Fan Ye1
1Department of Human Anatomy, West China School of Basic Medical Sciences and Forensic Medicine, Development and Related Diseases of Women and Children Key Laboratory of Sichuan Province, West China Second University Hospital, Sichuan University, Chengdu, Sichuan, China.
Gene Expression Patterns : GEP
|April 7, 2025
Summary
Mouse ovarian lymphangiogenesis begins postnatally on day 7, developing from the medulla to the cortex. This lymphatic vessel formation coincides with follicular maturation, involving YAP1 expression changes.
Area of Science:
- Reproductive biology
- Vascular biology
- Developmental biology
Background:
- Ovarian blood vasculature is well-studied, but ovarian lymphatic vasculature remains under-investigated.
- Limited studies have characterized ovarian lymphatic vessel formation.
Purpose of the Study:
- To investigate the morphological patterns of lymphatic vessel formation in mouse embryonic and early postnatal ovaries.
- To correlate ovarian lymphangiogenesis with follicular development and YAP1 expression.
Main Methods:
- Immunostaining of ovarian tissues from Prox1-EGFP transgenic and wild-type mice.
- Use of CD31 for vascular endothelium and LYVE1/Prox1 as lymphatic endothelium co-markers.
- Analysis of YAP1 expression in granulosa cells during ovarian development.
Main Results:
- Lymphatic markers (Prox1, LYVE1) and vessels were absent in the medulla until postnatal day 7 (P7).
- By P10, lymphatic markers were abundant in the medulla, and by P14, they extended to the cortex.
- YAP1 expression shifted from cytoplasm to nucleus in granulosa cells, correlating with follicular maturation stages (primary to antral and preovulatory).
Conclusions:
- Ovarian lymphangiogenesis in mice initiates on P7 and progresses from medulla to cortex.
- Lymphangiogenesis is synchronized with ovarian follicular maturation.
- YAP1 exhibits an ectopic expression pattern during ovarian lymphangiogenesis and follicular development.
