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Updated: May 15, 2025
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A Non-random Mouse Model for Pharmacological Reactivation of Mecp2 on the Inactive X Chromosome
Published on: May 22, 2019
6.3K
X-Linked Cancer-Associated Polypeptide (XCP) from lncRNA1456 Cooperates with PHF8 to Regulate Gene Expression and
Shrikanth S Gadad1,2,3,4, Cristel V Camacho1,2,4, Xuan Gong1,5
1Laboratory of Signaling and Gene Regulation, Cecil H. and Ida Green Center for Reproductive Biology Sciences, University of Texas Southwestern Medical Center, Dallas, TX 75390, USA.
Biorxiv : the Preprint Server for Biology
|April 8, 2025
Summary
Researchers discovered XCP, a protein encoded by a previously noncoding RNA (lncRNA1456), in breast cancer cells. XCP regulates cancer cell growth and interacts with PHF8 to control gene expression, highlighting new roles for noncoding RNAs.
Area of Science:
- Molecular Biology
- Genetics
- Oncology
Background:
- Long noncoding RNAs (lncRNAs) are increasingly recognized for producing functional proteins.
- The transcript lncRNA1456 (RHOXF1P3) was previously classified as noncoding.
Purpose of the Study:
- To identify and characterize novel functional proteins encoded by lncRNAs.
- To investigate the role of the newly discovered XCP protein in breast cancer.
Main Methods:
- Identification of XCP protein from human breast cancer cells.
- Analysis of lncRNA1456 and XCP expression in breast cancer subtypes.
- Functional studies in cell and xenograft models to assess XCP's role in cancer growth.
- Investigation of XCP's interaction with PHF8 and its mechanism of action.
Main Results:
- Discovery of XCP, a 132 amino acid protein encoded by the lncRNA1456.
- Upregulation of lncRNA1456 and XCP in luminal A, luminal B, and HER2 breast cancer subtypes.
- XCP modulates both estrogen-dependent and -independent breast cancer cell growth.
- XCP interacts with PHF8, enhancing its histone demethylase activity to regulate gene expression.
Conclusions:
- XCP is a functional protein derived from a lncRNA, implicated in breast cancer progression.
- XCP acts as a transcriptional co-regulator by modulating PHF8 activity.
- This study underscores the importance of exploring open reading frames in noncoding RNAs for potential therapeutic targets.
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