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A Protocol for High-efficiency Transformation and Genome Editing in Elite Wheat Cultivars
Karminderbir Kaur1, Akshaya K Biswal2, Rajvinder Kaur1
1Department of Plant Science, McGill University, Ste Anne de Bellevue, QC, Canada.
Abstract:
CRISPR-Cas9-based editing offers a simple yet efficient method for introducing targeted mutations in the genes of interest. Low efficiency of transformation and a lack of reproducibility in editing genes in elite wheat cultivars are the bottlenecks in improving simply inherited traits. We describe a high-efficiency transformation protocol for editing elite wheat cultivars using JD633-GRF4-GIF1 CRISPR vector. This vector reduces the regeneration time of transformants to less than 90 days and enables transformation of elite cultivars.
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