Evolutionary insights into adaptation of hemocyanins from deep-sea hydrothermal vent shrimps

Deep-sea hydrothermal vent shrimps inhabit environments with low oxygen levels and may even be exposed to hypoxic conditions. In response, their respiratory pigment, hemocyanin (Hc) may undergo molecular adaptations to enable them to survive in such extreme ecosystems. Therefore, we sampled four Alvinocarididae species from hydrothermal vents in the northern Central Indian Ridge and two types of Hc genes (α and γ) were observed. Employing the branch model, we detected positive selection for the deep-sea hydrothermal vent lineage, including 11 Decapoda species. Furthermore, using the branch-site model, we identified a putative mutant residue (Leu226, Ser377, and Ile390) close to the active site of Hc. Moreover, our results suggested potential molecular docking between two α-type Hc proteins. Thus, this study provides valuable and novel perspectives on the functional significance of the Hc gene in deep-sea hydrothermal vent shrimps, laying the foundation for future investigations in this intriguing area of research.