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Updated: May 8, 2025

Avian Semen Collection by Cloacal Massage and Isolation of DNA from Sperm
Published on: February 5, 2018
Semen Extraction and Management in Wild Animals: TUMASG Technique
Julián Santiago-Moreno1, Adolfo Toledano-Díaz2, Cristina Castaño2
1Department of Animal Reproduction, INIA-CSIC, Madrid, Spain. moreno@inia.csic.es.
Abstract:
Transrectal ultrasound-guided massage of the accessory sex glands (TUMASG) is a technique to collect semen that is less stressful than electroejaculation. TUMASG is useful in obtaining sperm samples from wild species, requiring few subsequent electrical electroejaculation stimuli and sometimes none at all. The ejaculatory response to TUMAG is species-specific, and maximum effectiveness is found in ruminants. Because certain anesthetics can interfere with the neuromuscular mechanisms that control erectile and ejaculatory functions, an appropriate anesthetic combination is needed if an adequate ejaculatory response is to be obtained. Here, we describe the technique TUMASG, the better anesthetic combination, and semen management to use in wild ruminants.

