Related Experiment Video
Updated: May 8, 2025
A High-Throughput Method For Zebrafish Sperm Cryopreservation and In Vitro Fertilization
Published on: July 6, 2009
Rabbit Sperm Cryopreservation
Maria Pilar Viudes de Castro1, Francisco Marco-Jimenez2, Jose Salvador Vicente2
1Centro de Investigación y Tecnología Animal, Instituto Valenciano de Investigaciones Agrarias (CITA-IVIA), 12400 Segorbe (Castellón), Spain. viudes_mar@gva.es.
Abstract:
Sperm cryopreservation is an excellent way to store genetic materials for a long period. Semen cryopreservation is associated with structural and functional damage of the spermatozoa caused by cold shock, osmotic stress, intracellular ice crystal formation, and the production of reactive oxygen species, which reduce their functional lifespan and fertilization capacity. Here, we describe the main rabbit sperm cryopreservation protocol.
