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Oxidative Status Measurement in Domestic and Wild Small Ruminants' Species.

Olga García-Álvarez1, Ana Josefa Soler2, María Iniesta-Cuerda3,4

  • 1Grupo SaBio. IREC (UCLM-CSIC-JCCM). ETSIAMB, Albacete, Spain. olga.garcia@uclm.es.

Methods in Molecular Biology (Clifton, N.J.)
|April 9, 2025
PubMed
Summary

Oxidative stress damages sperm biomolecules, leading to male infertility. This study details flow cytometry methods to assess oxidative stress effects on small ruminant sperm, combining fluorescent probes with immunodetection.

Keywords:
Antioxidant enzymesDNA damageFlow cytometryLipid peroxidationOxidative stressROS productionSperm damage

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Area of Science:

  • Reproductive biology
  • Oxidative stress research
  • Animal science

Background:

  • Oxidative stress causes significant damage to sperm biomolecules, DNA, lipids, carbohydrates, and proteins.
  • This damage disrupts cellular components and signaling pathways, ultimately impairing male fertility.
  • Small ruminants, both domestic and wild, are susceptible to these effects, impacting reproductive success.

Purpose of the Study:

  • To describe methodologies for evaluating oxidative stress impacts on sperm attributes in small ruminants.
  • To present advanced techniques combining flow cytometry with immunodetection for detailed analysis.
  • To provide a comprehensive guide for assessing sperm quality under oxidative stress conditions.

Main Methods:

  • Utilizing flow cytometry as the primary analytical technique.
  • Employing traditional fluorescent probes to detect cellular damage and viability.
  • Integrating immunodetection methods with flow cytometry for specific molecular analysis.

Main Results:

  • The described methods allow for precise quantification of oxidative stress-induced damage in sperm.
  • Combining fluorescent probes and immunodetection enhances the sensitivity and specificity of the analysis.
  • The techniques are applicable to both domestic and wild small ruminant species.

Conclusions:

  • Flow cytometry, especially when combined with immunodetection, is a powerful tool for assessing oxidative stress in sperm.
  • These methods are crucial for understanding male sub/infertility causes in small ruminants.
  • The chapter provides valuable insights and practical protocols for researchers in reproductive biology and animal science.