Xylose isomerase: From fundamental research to applied enzyme technology

Xylose isomerases (XI, EC 5.3.1.5) are key enzymes for the metabolism of pentoses by microorganisms. The importance of XIs goes beyond academic biochemical research and the catalysis of aldo-ketose conversion by XIs is among the most successful examples of industrial enzyme technology in a market that generates multibillion dollar annual revenues. Here we present an in-depth review of how structural information has contributed to the current understanding of XI catalysis, and discuss topics related to the ongoing efforts to elucidate key aspects of the catalytic mechanism. An overview of XI immobilization is also provided that illustrates how the discoveries in basic enzyme technology research can generate opportunities for novel uses of XI, and we review not only historical aspects but also more recent applications in HFCS, biofuels and other applications. The systems biology revolution will impact all aspects of XI research and application, and we finalize by reviewing the contemporary efforts of metabolic and protein engineering using XI and the future roles of the enzyme in the expanding bioeconomy.