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Updated: May 15, 2025
06:02
Arbovirus Infections As Screening Tools for the Identification of Viral Immunomodulators and Host Antiviral Factors
Published on: September 13, 2018
6.8K
Omics Analyses Uncover Host Networks Defining Virus-Permissive and -Hostile Cellular States
Honglin Chen1, Philip D Charles2, Quan Gu3
1MRC-University of Glasgow Centre for Virus Research, Glasgow, UK; Department of Biochemistry, University of Oxford, Oxford, UK.
Molecular & Cellular Proteomics : MCP
|April 9, 2025
Summary
Host cell proteomes dictate virus infection outcomes. This study maps proteins and phosphorylation changes linked to cellular permissiveness, revealing key regulators of viral susceptibility.
Area of Science:
- Virology
- Cell Biology
- Proteomics
Background:
- Cellular proteomes significantly influence the ability of host cells to support or inhibit viral infections.
- Understanding the protein landscape of cellular permissiveness is crucial for addressing fundamental questions in virology.
Purpose of the Study:
- To comprehensively map proteins associated with highly permissive, intermediate, and hostile cellular states using a multi-omic approach.
- To identify regulatory mechanisms, including protein abundance and phosphorylation, that govern viral susceptibility.
Main Methods:
- Multi-omic analysis integrating transcriptomics and proteomics.
- Phosphoproteomics to identify altered phosphorylation patterns.
- Overexpression screens to assess antiviral effects of identified proteins.
Main Results:
- Two distinct groups of differentially regulated genes were identified: those with concordant mRNA/protein changes and those with discordant protein/RNA levels.
- Many interferon-stimulated genes (ISGs) with discordant profiles lacked antiviral activity in screens.
- Altered phosphorylation of non-signaling proteins represents an additional regulatory layer.
- Several identified permissiveness-associated proteins, varying in abundance or phosphorylation, were confirmed to regulate infection fitness.
Conclusions:
- Cellular protein abundance and phosphorylation status are critical determinants of viral susceptibility.
- Protein-level changes, particularly those influenced by interferon signaling, may be more indicative of antiviral function than mRNA levels.
- This study provides a systematic map of cellular alterations impacting virus infection outcomes.
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