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Related Concept Videos

Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing02:18

Alternative RNA Splicing

Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing01:32

RNA Splicing

Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Editing02:23

RNA Editing

RNA editing is a post-transcriptional modification where a precursor mRNA (pre-mRNA) nucleotide sequence is changed by base insertion, deletion, or modification. The extent of RNA editing varies from a few hundred bases, in mitochondrial DNA of trypanosomes, to a just single base, in nuclear genes of mammals. Even a single base change in the pre-mRNA can convert a codon for one amino acid into the codon for another amino acid or a stop codon. This type of re-coding can significantly affect the...
Chromatin Structure Regulates pre-mRNA Processing02:41

Chromatin Structure Regulates pre-mRNA Processing

In eukaryotic cells, nascent mRNA transcripts need to undergo many post-transcriptional modifications to reach the cell cytoplasm and translate into functional proteins. For a long time, transcription and pre-mRNA processing were considered two independent events that occur sequentially in the cell. However, it has now been well established that transcription and pre-mRNA processing are two simultaneous processes that are precisely regulated inside the cell.
The chromatin structure, especially...

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Updated: May 14, 2026

Using the E1A Minigene Tool to Study mRNA Splicing Changes
10:25

Using the E1A Minigene Tool to Study mRNA Splicing Changes

Published on: April 22, 2021

ADAR1 Regulates Alternative Splicing Through an RNA Editing-Independent Mechanism.

Eduardo A Sagredo1,2, Victor Karlström1, Alejandro Blanco3

  • 1Department of Molecular Biosciences, The Wenner-Gren Institute, Stockholm University, Svante Arrhenius 20C, 106 91 Stockholm, Sweden.

International Journal of Molecular Sciences
|May 13, 2026
PubMed
Summary
This summary is machine-generated.

The RNA-editing enzyme ADAR1p110 isoform regulates alternative splicing independently of its editing function. This non-canonical role impacts therapeutic response genes, suggesting new cancer treatment strategies.

Keywords:
ACIN1ADAR1RNA editingalternative splicingepitranscriptomicsproteomicsspliceosome

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Area of Science:

  • Molecular Biology
  • Genetics
  • Biochemistry

Background:

  • Dysregulated ADAR1 is linked to cancer, but its non-editing functions in alternative splicing are poorly understood.
  • Understanding ADAR1's alternative splicing roles may reveal novel therapeutic targets for diseases like cancer.

Purpose of the Study:

  • To investigate the RNA-editing-independent mechanisms by which the ADAR1p110 isoform influences alternative splicing.
  • To explore the impact of ADAR1 on the splicing landscape and its implications for therapeutic response in cancer.

Main Methods:

  • RNA-sequencing and proteomic analysis of ADAR1p110 interactors.
  • Functional assays using wild-type and mutant ADAR1 in various human cell lines, including cancer models.
  • Investigated ADAR1's interaction with spliceosome components and splicing regulators.

Main Results:

  • ADAR1p110 interacts with key spliceosome components and splicing regulators.
  • ADAR1 significantly alters alternative splicing events, largely independent of its RNA-editing or RNA-binding activity.
  • ADAR1 influences the expression of other splicing factors, indicating indirect regulatory roles.
  • This splicing reprogramming affects genes critical for therapeutic response, potentially driving drug resistance.

Conclusions:

  • ADAR1 possesses significant RNA-editing-independent functions in regulating alternative splicing.
  • The ADAR-splicing axis represents a potential mechanism for drug resistance in cancer.
  • This study uncovers non-canonical ADAR1 functions, offering new therapeutic avenues for cancer treatment.