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Qualitative and quantitative structural changes during pig oocyte maturation.

D G Cran

    Journal of Reproduction and Fertility
    |May 1, 1985
    PubMed
    Summary

    Hormone stimulation triggers pig oocyte maturation, marked by mitochondrial fusion and organelle redistribution. This process is crucial for developmental competence in porcine oocytes.

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    Area of Science:

    • Reproductive Biology
    • Cell Biology
    • Developmental Biology

    Background:

    • Oocyte maturation is a complex process involving significant cellular reorganization.
    • Understanding organelle dynamics during maturation is key to improving assisted reproductive technologies.

    Purpose of the Study:

    • To investigate the temporal changes in organelle distribution and morphology in pig oocytes following human chorionic gonadotropin (hCG) stimulation.
    • To elucidate the role of mitochondrial dynamics and interactions with the endoplasmic reticulum (ER) during oocyte maturation.

    Main Methods:

    • Pig oocytes were collected and stimulated with hCG.
    • Oocytes were analyzed at hourly intervals to track morphological and organelle changes.
    • Mitochondrial distribution, lipid droplet formation, and vesicle volume were quantified.

    Main Results:

    • Meiosis resumption occurred 20–30 hours post-hCG, coinciding with decreased mitochondrial numbers, likely due to fusion.
    • An increase in lipid droplets and a decrease in the volume fraction of large vesicles were observed.
    • Mitochondria dispersed from the periphery, and large vesicles, protein bodies, and Golgi decreased at the oocyte edge during maturation.

    Conclusions:

    • Hormonal stimulation induces significant organelle remodeling in pig oocytes.
    • Mitochondrial fusion and redistribution, along with changes in lipid droplets and vesicles, are integral to porcine oocyte maturation.

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