Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Modular cardiac microtissues for interrogating cell-type contributions.

Nature reviews. Cardiology·2026
Same author

Frataxin deficiency drives cardiac dysfunction and transcriptional dysregulation in Friedreich ataxia iPSC model.

Cell death & disease·2026
Same author

Long-read sequencing-based atlas of tissue-specific expression of DNM1L transcript variants.

The FEBS journal·2026
Same author

Evaluation of CRISPR/CasRx-Mediated VEGF mRNA Knockdown in Mouse Retina.

Methods in molecular biology (Clifton, N.J.)·2026
Same author

Hypoxia Modulates Nanovesicle Phenotype and Angiogenic Function From Human Pluripotent Stem Cells.

Proteomics·2026
Same author

Friedreich ataxia transcriptomic dysregulation and identification of cell type-specific biomarkers: A systematic review and meta-analysis.

bioRxiv : the preprint server for biology·2026
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: May 8, 2025

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels
07:49

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

3.4K

Methods for Assessing Scaffold Vascularization with Human Endothelial Cells.

Anne M Kong1, Guei-Sheung Liu2,3,4, Geraldine M Mitchell1,5,6

  • 1O'Brien Institute Department, St Vincent's Institute of Medical Research, Fitzroy, VIC, Australia.

Methods in Molecular Biology (Clifton, N.J.)
|April 10, 2025
PubMed
Summary
This summary is machine-generated.

This study developed a method to create vascularized tissue constructs for regenerative medicine. By pre-vascularizing scaffolds with endothelial cells, the approach enhances tissue integration and functionality.

Keywords:
Endothelial cellsExtrinsic vascularizationIntrinsic vascularizationScaffold vascularizationTissue engineering

More Related Videos

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
05:52

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Published on: September 27, 2019

9.3K
Author Spotlight: Investigating Angiogenesis Through Challenges and Innovations in Assay Development
09:16

Author Spotlight: Investigating Angiogenesis Through Challenges and Innovations in Assay Development

Published on: May 31, 2024

1.0K

Related Experiment Videos

Last Updated: May 8, 2025

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels
07:49

Stepwise Cell Seeding on Tessellated Scaffolds to Study Sprouting Blood Vessels

Published on: January 14, 2021

3.4K
Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures
05:52

Core/shell Printing Scaffolds For Tissue Engineering Of Tubular Structures

Published on: September 27, 2019

9.3K
Author Spotlight: Investigating Angiogenesis Through Challenges and Innovations in Assay Development
09:16

Author Spotlight: Investigating Angiogenesis Through Challenges and Innovations in Assay Development

Published on: May 31, 2024

1.0K

Area of Science:

  • Biomaterials Science
  • Regenerative Medicine
  • Stem Cell Biology

Background:

  • Successful tissue engineering requires efficient vascularization for nutrient supply and waste removal.
  • Existing scaffolds often lack the necessary support for vascular cell survival and capillary network formation.
  • In vivo vascularization is critical for the integration and long-term function of engineered tissues.

Purpose of the Study:

  • To develop a method for generating pre-vascularized scaffolds using endothelial cells derived from induced pluripotent stem cells.
  • To evaluate the efficacy of these pre-vascularized scaffolds in creating functional vascularized tissue constructs in vivo.
  • To advance the potential of tissue engineering for clinical applications and drug development.

Main Methods:

  • Generating endothelial cells from induced pluripotent stem cells (iPSCs).
  • Seeding endothelial cells into 3D porous scaffolds to create pre-vascularized constructs.
  • Implanting pre-vascularized scaffolds in a rat subcutaneous model for in vivo evaluation.

Main Results:

  • Demonstrated successful generation of endothelial cells from iPSCs.
  • Showcased the ability to pre-vascularize 3D porous scaffolds.
  • Established a model for creating vascularized tissue constructs via in vivo implantation.

Conclusions:

  • The presented method enables the creation of pre-vascularized scaffolds, a key step towards functional tissue engineering.
  • This approach shows significant promise for improving the vascularization of engineered tissues for regenerative medicine.
  • The developed technique could accelerate the translation of tissue-engineered constructs into clinical practice and drug discovery pipelines.