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DNA-Tethered RNA Polymerase for Programmable In vitro Transcription and Molecular Computation
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Innovations, Challenges and Future Directions of T7RNA Polymerase in Microbial Cell Factories

Sefli Sri Wahyu Effendi1, I-Son Ng1

  • 1Department of Chemical Engineering, National Cheng Kung University, Tainan 701, Taiwan.

ACS Synthetic Biology
|April 10, 2025
PubMed
Summary

Bacteriophage T7 RNA polymerase (T7RNAP) is key for optimizing microbial cell factories. This review guides T7RNAP variant use in synthetic biology and bioproduction, highlighting advancements and future opportunities.

Keywords:
T7RNAPcircuit designgene regulationgenetic engineeringmicrobial cell factory

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