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Related Experiment Video

Updated: Jun 25, 2026

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Subcloning Plus Insertion SPI - A Novel Recombineering Method for the Rapid Construction of Gene Targeting Vectors

Published on: January 8, 2015

It's prime time for multiplexed prime editing.

Ke Wu¹, Francisco J Sánchez-Rivera¹

¹David H. Koch Institute for Integrative Cancer Research, Massachusetts Institute of Technology, Cambridge, MA 02142, USA; Department of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142, USA.

|April 10, 2025

Summary

This summary is machine-generated.

Prime editing screens enable precise genetic variant studies but face efficiency challenges. Researchers optimized these screens to investigate variants in SMARCB1 and MLH1 genes.

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Last Updated: Jun 25, 2026

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Published on: January 8, 2015

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CRISPR/Cas9 Editing of the C. elegans rbm-3.2 Gene using the dpy-10 Co-CRISPR Screening Marker and Assembled Ribonucleoprotein Complexes.

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Published on: December 11, 2020

Area of Science:

Genetics
Molecular Biology
Genomic Engineering

Background:

Prime editing screens offer precise, scalable genetic variant analysis in native contexts.
Variable editing efficiency currently limits the application of prime editing screens.

Purpose of the Study:

To overcome the limitations of variable editing efficiency in prime editing screens.
To apply optimized prime editing screens for investigating genetic variants in SMARCB1 and MLH1.

Main Methods:

Optimization of prime editing screen protocols.
Application of enhanced prime editing screens to study specific genes.

Main Results:

Successfully improved and standardized prime editing efficiency.
Generated valuable data on variants within the SMARCB1 and MLH1 genes.

Conclusions:

Optimized prime editing screens are a powerful tool for genetic variant research.
This approach enhances the study of genes like SMARCB1 and MLH1, crucial for various biological processes.