Immuno-PCR: A high-sensitivity approach for biomarker analysis

The integration of immunology and molecular biology, Immuno-Polymerase Chain Reaction, ie, Immuno-PCR, (iPCR), is an innovative cutting-edge detection strategy that holds significant promise for the identification of pathophysiologic biomarkers present at very low concentration or those inherently unstable. iPCR, known for superior sensitivity and specificity, has proven valuable in early disease diagnosis, monitoring and prognosis. This review summarizes the current applications of iPCR in detecting various disease biomarkers including those related to cancer, infection, autoimmune, cardiovascular, and neurological disease. We introduce the principle, advantages and limitations, specific applications, and clinical significance of iPCR, thereby promoting the widespread application of this technology in disease diagnosis. This technology facilitates early detection and intervention, enhances patient outcomes and survival rates, and is a valuable reference for future research and applications.