Genetic characterization of coronaviruses causing common cold symptoms based on micro-targeted capture sequencing

A novel micro-targeted capture sequencing method was developed for four coronaviruses that cause common cold symptoms, and its sensitivity and clinical performance were assessed using 14 pharyngeal swab samples. This technique achieved 100% genomic coverage for samples with a cycle threshold (Ct) value of 32 or lower. The genotypes of the clinical isolates were determined by phylogenetic analysis, and unique mutations were identified in the receptor-binding domain (RBD) of the spike protein. Additional N-glycosylation sites were found in some of the isolates. This method offers a sensitive and rapid tool for genetic monitoring of common-cold coronaviruses and aids in their prevention and control.