Related Experiment Video
Updated: May 15, 2025
09:36
Unbiased Deep Sequencing of RNA Viruses from Clinical Samples
Published on: July 2, 2016
16.8K
Genetic characterization of coronaviruses causing common cold symptoms based on micro-targeted capture sequencing
Qiao Qiao1, Xiaojuan Zhu1, Zixinrong Meng2
1NHC Key Laboratory of Enteric Pathogenic Microbiology, Jiangsu Provincial Medical Key Laboratory of Pathogenic Microbiology in Emerging Major Infectious Diseases, Jiangsu Provincial Center for Disease Control and Prevention, Nanjing, 210009, China.
Archives of Virology
|April 10, 2025
Summary
A new sequencing method accurately detects common cold coronaviruses. This rapid genetic monitoring tool aids in preventing and controlling these prevalent respiratory viruses.
Area of Science:
- Virology
- Genomics
- Molecular Biology
Background:
- Common cold symptoms are frequently caused by four distinct coronaviruses.
- Effective genetic monitoring is crucial for understanding and controlling viral spread.
Purpose of the Study:
- To develop and evaluate a novel micro-targeted capture sequencing method for common cold coronaviruses.
- To assess the sensitivity and clinical performance of this new technique.
Main Methods:
- Development of a micro-targeted capture sequencing assay.
- Application of the assay to 14 clinical pharyngeal swab samples.
- Phylogenetic analysis for genotype determination and mutation identification.
Main Results:
- The method achieved 100% genomic coverage for samples with Ct values ≤ 32.
- Unique mutations in the spike protein's receptor-binding domain (RBD) were identified.
- Additional N-glycosylation sites were detected in some isolates.
Conclusions:
- The developed method is a sensitive and rapid tool for genetic surveillance of common cold coronaviruses.
- This technique can significantly aid in the prevention and control strategies for these viruses.
Related Concept Videos
01:05Single Nucleotide Polymorphisms-SNPs
13.7K
A single nucleotide polymorphism or SNP is a single nucleotide variation at a specific genomic position in a large population. It is the most prevalent type of sequence variation found in the human genome. Point mutations that occur in more than 1% of the population qualify as SNPs. These are present once every 1000 nucleotides on an average in the human genome. Replacement of a purine with another purine (A/G) or a pyrimidine with another pyrimidine (C/T) is known as a transition. In contrast,...
13.7K
03:21RNA-seq
9.7K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases.
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.7K