Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

lncRNA - Long Non-coding RNAs02:39

lncRNA - Long Non-coding RNAs

8.4K
In humans, more than 80% of the genome gets transcribed. However, only around 2% of the genome codes for proteins. The remaining part produces non-coding RNAs which includes ribosomal RNAs, transfer RNAs, telomerase RNAs, and regulatory RNAs, among other types. A large number of regulatory non-coding RNAs have been classified into two groups depending upon their length – small non-coding RNAs, such as microRNA, which are less than 200 nucleotides in length, and long non-coding RNA...
8.4K
Types of RNA01:20

Types of RNA

5.5K
Three main types of RNA are involved in protein synthesis: messenger RNA (mRNA), transfer RNA (tRNA), and ribosomal RNA (rRNA). These RNAs perform diverse functions and can be broadly classified as protein-coding or non-coding RNA. Non-coding RNAs play important roles in regulating gene expression in response to developmental and environmental changes. Non-coding RNAs in prokaryotes can be manipulated to develop more effective antibacterial drugs for human or animal use.
RNA Performs Diverse...
5.5K
Ribosomal RNA Synthesis02:53

Ribosomal RNA Synthesis

13.0K
Ribosome synthesis is a highly complex and coordinated process involving more than 200 assembly factors. The synthesis and processing of ribosomal components occurs not only in the nucleolus but also in the nucleoplasm and the cytoplasm of eukaryotic cells.
Ribosome biogenesis begins with the synthesis of 5S and 45S pre-rRNAs by distinct RNA polymerases. The primary transcripts are extensively processed and modified before they are bound and folded by ribosomal proteins and assembly factors,...
13.0K
RNA Interference01:23

RNA Interference

25.8K
RNA interference (RNAi) is a process in which a small non-coding RNA molecule blocks the post-transcriptional expression of a gene by binding to its messenger RNA (mRNA) and preventing the protein from being translated.
This process occurs naturally in cells, often through the activity of genomically-encoded microRNAs. Researchers can take advantage of this mechanism by introducing synthetic RNAs to deactivate specific genes for research or therapeutic purposes. For example, RNAi could be used...
25.8K
Nucleic Acids02:43

Nucleic Acids

43.1K
Nucleic acids are the most important macromolecules for the continuity of life. They carry the cell's genetic blueprint and carry instructions for its functioning.
DNA and RNA
The two main types of nucleic acids are deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). DNA is the genetic material in all living organisms, ranging from single-celled bacteria to multicellular mammals. It is in the nucleus of eukaryotes and in the organelles, chloroplasts, and mitochondria. In prokaryotes,...
43.1K
MicroRNAs01:22

MicroRNAs

21.1K
MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
21.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Preoperative Plasma and Cerebrospinal Fluid SNAP-25 Levels Predict Delayed Neurocognitive Recovery in Elderly Patients: A Prospective Observational Study.

CNS neuroscience & therapeutics·2026
Same author

The Role of β-Klotho in FGF Signaling: From Molecular Insights to Therapeutic Innovations.

American journal of physiology. Endocrinology and metabolism·2026
Same author

RGTBind: RBF-gate graph transformer with spatially biased attention for protein-DNA binding-site prediction.

Journal of molecular modeling·2026
Same author

Oncofetal RNA-binding proteins of IGF2BP family suppress IRF-3 and NF-kB dependent transcription downstream of cytosolic RNA sensors.

bioRxiv : the preprint server for biology·2026
Same author

Estrogen Deprivation and Periodontitis Interact Across Multiple Tissues.

bioRxiv : the preprint server for biology·2026
Same author

The mediating role of self-efficacy between time management disposition and deep learning ability among undergraduate nursing students: A cross-sectional study.

Nurse education today·2026

Related Experiment Video

Updated: May 15, 2025

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

23.9K

Noncoding RNA, ncRNA-a3, Epigenetically Regulates TAL1 Transcriptional Program During Erythropoiesis.

Meghana Matur1, Yasin Uzun1,2, Xiangguo Shi1

  • 1Division of Pediatric Hematology/Oncology, Department of Pediatrics, Pennsylvania State University College of Medicine, Hershey, Pennsylvania, USA.

Molecular and Cellular Biology
|April 11, 2025
PubMed
Summary

A novel enhancer RNA, ncRNA-a3, is crucial for activating TAL1 transcription during red blood cell development. Its loss impairs erythroid differentiation by disrupting chromatin interactions and accessibility at the TAL1 locus.

Keywords:
TAL1 oncogenechromatin structureeRNAerythropoiesisgene regulation

More Related Videos

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

9.0K
MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

6.6K

Related Experiment Videos

Last Updated: May 15, 2025

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster
09:39

Enhanced Northern Blot Detection of Small RNA Species in Drosophila Melanogaster

Published on: August 21, 2014

23.9K
Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution
10:53

Isolation of Cognate RNA-protein Complexes from Cells Using Oligonucleotide-directed Elution

Published on: January 16, 2017

9.0K
MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria
08:34

MS2-Affinity Purification Coupled with RNA Sequencing in Gram-Positive Bacteria

Published on: February 23, 2021

6.6K

Area of Science:

  • Hematopoiesis research
  • Epigenetics and non-coding RNA biology
  • Transcriptional regulation

Background:

  • Hematopoietic transcription involves complex interactions of transcription factors, chromatin modifiers, and non-coding RNAs.
  • TAL1 is a key regulator in both normal and malignant hematopoiesis, but its precise regulation during erythropoiesis and leukemogenesis is not fully understood.

Purpose of the Study:

  • To elucidate the mechanism regulating TAL1 activity during erythropoiesis.
  • To investigate the role of the enhancer RNA ncRNA-a3 in TAL1 activation and erythroid differentiation.

Main Methods:

  • Analysis of ncRNA-a3 transcription and its correlation with TAL1 locus chromatin accessibility.
  • Functional studies involving loss of ncRNA-a3 in hematopoietic stem and progenitor cells.
  • Assessment of erythroid differentiation and rescue experiments with TAL1 cDNA overexpression.
  • Investigation of ncRNA-a3's role in chromatin interactions and recruitment of epigenetic modifiers (p300/BRG1).

Main Results:

  • ncRNA-a3 is positively correlated with TAL1 locus chromatin accessibility and transcription.
  • Loss of ncRNA-a3 impairs TAL1 activation and terminal erythroid differentiation.
  • ncRNA-a3 facilitates long-range chromatin interactions, maintaining the TAL1 locus as an erythroid interaction hub.
  • ncRNA-a3 promotes p300/BRG1 recruitment, enhancing chromatin accessibility and TAL1 transcription.

Conclusions:

  • ncRNA-a3 plays a novel and essential role in TAL1-dependent erythropoiesis.
  • ncRNA-a3 establishes a new mechanism for regulating TAL1 transcriptional activation through enhancer RNA function.