Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

3.2K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
3.2K
Preparation of Samples for Electron Microscopy01:20

Preparation of Samples for Electron Microscopy

5.3K
To be visualized by an electron microscope, either transmission or scanning, biological samples need to be fixed (stabilized) so the electron beam does not destroy them and dried thoroughly (desiccated/dehydrated) so the vacuum does not affect them. Fixation needs to be done as quickly as possible because the sample properties will start changing as soon as it is removed from its natural environment. For example, in a tissue sample, the oxygen levels begin decreasing, causing an altered...
5.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

An Inorganic Layered Coordination Polymer as High-Performance Solid-State Electrolyte for Stable Lithium Metal Batteries.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Tailoring the Work Function of Oxyhalide Solid Electrolytes via Sulfur Doping to Boost High-Performance All-Solid-State Lithium Batteries.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Harnessing High-Pressure CO<sub>2</sub> for Molecular-Scale Interfacial Engineering in Sulfide-Based All‑Solid‑State Lithium Metal Batteries.

Advanced materials (Deerfield Beach, Fla.)·2026
Same author

Facet-engineered Prussian blue analog nanosheet-assembled superstructures for efficient syngas production.

Chemical communications (Cambridge, England)·2026
Same author

Mechanistic insights into high performance W<sup>6+</sup>-doped Li<sub>3</sub>YCl<sub>6</sub> solid state electrolytes: synergy of vacancies and lattice softening.

Physical chemistry chemical physics : PCCP·2026
Same author

A Universal AgP<sub>2</sub> Nanowire Modification Method Enabling Durable Anodes for Alkaline Seawater Electrolysis.

ACS applied materials & interfaces·2026

Related Experiment Video

Updated: May 15, 2025

Nanoscale Characterization of Liquid-Solid Interfaces by Coupling Cryo-Focused Ion Beam Milling with Scanning Electron Microscopy and Spectroscopy
11:03

Nanoscale Characterization of Liquid-Solid Interfaces by Coupling Cryo-Focused Ion Beam Milling with Scanning Electron Microscopy and Spectroscopy

Published on: July 14, 2022

3.4K

Cryo-Electron Microscopy for Unveiling the Sensitive Battery Materials.

Zhijin Ju1, Huadong Yuan1, Ouwei Sheng1

  • 1College of Materials Science and Engineering Zhejiang University of Technology Hangzhou 310014 China.

Small Science
|April 11, 2025
PubMed
Summary
This summary is machine-generated.

Cryo-electron microscopy (cryo-EM) enables high-resolution imaging of reactive lithium metal battery components in their native state. This technique is crucial for understanding lithium dendrites and interface chemistry for advanced energy storage.

Keywords:
battery materialscryo-electron microscopyelectrolyte interphaselithium metal anodelithium metal batteries

More Related Videos

Failure Analysis of Batteries Using Synchrotron-based Hard X-ray Microtomography
08:11

Failure Analysis of Batteries Using Synchrotron-based Hard X-ray Microtomography

Published on: August 26, 2015

8.8K
Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering
07:55

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering

Published on: April 17, 2018

12.6K

Related Experiment Videos

Last Updated: May 15, 2025

Nanoscale Characterization of Liquid-Solid Interfaces by Coupling Cryo-Focused Ion Beam Milling with Scanning Electron Microscopy and Spectroscopy
11:03

Nanoscale Characterization of Liquid-Solid Interfaces by Coupling Cryo-Focused Ion Beam Milling with Scanning Electron Microscopy and Spectroscopy

Published on: July 14, 2022

3.4K
Failure Analysis of Batteries Using Synchrotron-based Hard X-ray Microtomography
08:11

Failure Analysis of Batteries Using Synchrotron-based Hard X-ray Microtomography

Published on: August 26, 2015

8.8K
Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering
07:55

Elemental-sensitive Detection of the Chemistry in Batteries through Soft X-ray Absorption Spectroscopy and Resonant Inelastic X-ray Scattering

Published on: April 17, 2018

12.6K

Area of Science:

  • Materials Science
  • Electrochemistry
  • Microscopy

Background:

  • Investigating next-generation high energy density batteries requires deep chemical and structural analysis of components.
  • Lithium (Li) metal anodes and their solid electrolyte interphase (SEI) layers are reactive and sensitive to electron-beam irradiation, hindering traditional high-resolution imaging.
  • Understanding these materials is vital for developing robust and high-performance rechargeable batteries.

Purpose of the Study:

  • To review the contributions of cryo-electron microscopy (cryo-EM) to the characterization of sensitive battery materials.
  • To highlight the potential of cryo-EM for revealing physicochemical properties of energy materials at the nanoscale.
  • To provide insights into the development of cryo-EM for future battery research.

Main Methods:

  • Application of cryo-electron microscopy (cryo-EM) for high-resolution imaging of battery materials.
  • Preservation of native sample states during imaging through cryogenic techniques.
  • Classification of cryo-EM contributions: visualization of Li dendrites, inactive Li, and electrode interface chemistry.

Main Results:

  • Cryo-EM allows for high-resolution imaging of Li metal and SEI layers without degradation.
  • Visualization of critical features like Li dendrites and inactive Li is achieved at nanometer to atomic scales.
  • Cryo-EM facilitates the study of electrode interface chemistry in its native environment.

Conclusions:

  • Cryo-EM is a powerful tool for in-depth characterization of sensitive battery materials.
  • This technique is essential for understanding the fundamental properties governing battery performance.
  • Future developments in cryo-EM will further advance the field of high-performance rechargeable batteries.