Scriptaid Improves Cashmere Goat Embryo Reprogramming by Affecting Donor Cell Pluripotency Molecule NANOG Expression.

Area of Science:

• Reproductive biology
• Epigenetics
• Animal biotechnology

Background:

• Somatic cell nuclear transfer (SCNT) efficiency is limited by reprogramming abnormalities in donor cells and reconstructed embryos.
• Histone deacetylase inhibitors (HDACi) can modify epigenetic marks to potentially improve SCNT outcomes.

Purpose of the Study:

• To investigate the effect of Scriptaid, an HDACi, on donor cell epigenetic modifications and SCNT efficiency in Albas cashmere goats.
• To assess the impact of Scriptaid treatment on histone acetylation, pluripotency gene expression, and embryo development.

Main Methods:

• Albas cashmere goat cells were treated with Scriptaid.
• SCNT embryos were reconstructed using treated donor cells.
• Histone acetylation levels, NANOG expression, cell proliferation, apoptosis, and cell cycle were analyzed.

Main Results:

• Scriptaid treatment significantly increased histone acetylation in donor cells.
• NANOG expression was perturbed, and reprogramming ability of embryos was altered.
• Scriptaid treatment inhibited donor cell proliferation, induced apoptosis, and caused G0/G1 cell cycle arrest.
• The developmental rate of SCNT-reconstructed embryos was increased.

Conclusions:

• Scriptaid treatment improves SCNT efficiency in goats by enhancing epigenetic reprogramming and embryo development.
• This approach offers a promising strategy for advancing regenerative medicine, agriculture, and animal husbandry through improved SCNT technology.