Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Reclone: a global research community building equitable access to reagents.

Nature methods·2026

Same author

Elucidating the ancestral role of Class I HD-Zip transcription factors in land plants.

Plant physiology·2026

Same author

MobileLAMP: A low-cost, portable incubation device for isothermal nucleic acid amplification.

PloS one·2026

Same author

Plant Synthetic Biology Takes Off: Bryophytes Onboard with New Tools, Systems, and Opportunities.

ACS synthetic biology·2026

Same author

A simple cell-cycle control system in Marchantia polymorpha provides a framework for understanding plant cell proliferation.

The Plant cell·2026

Same author

Community-building resources for Marchantia-an accessible model plant system.

Plant & cell physiology·2026

Search research articles

Related Experiment Video

Updated: May 14, 2025

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

Semi-automated workflow for high-throughput Agrobacterium-mediated plant transformation.

Davide Annese¹, Facundo Romani¹, Carolina Grandellis²

¹Department of Plant Sciences, University of Cambridge, Cambridge, UK.

The Plant Journal : for Cell and Molecular Biology

|April 12, 2025

Summary

We developed efficient protocols for plant transformation using Agrobacterium tumefaciens and Marchantia polymorpha. This accelerates high-throughput experiments in plant synthetic biology by reducing costs and time.

Keywords:

Agrobacterium tumefaciens Marchantia polymorpha stable transformation technical advance transient transformation

More Related Videos

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

Published on: September 27, 2016

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

Related Experiment Videos

Last Updated: May 14, 2025

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Generating Transgenic Plants with Single-copy Insertions Using BIBAC-GW Binary Vector

Published on: March 28, 2018

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

A Robotic Platform for High-throughput Protoplast Isolation and Transformation

Published on: September 27, 2016

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Inducible, Cell Type-Specific Expression in Arabidopsis thaliana Through LhGR-Mediated Trans-Activation

Published on: April 19, 2019

Area of Science:

Plant Biology
Synthetic Biology
Biotechnology

Background:

High-throughput plant experiments are limited by long generation times and high costs.
Developing efficient transformation protocols is crucial for advancing plant synthetic biology.

Purpose of the Study:

To optimize Agrobacterium tumefaciens transformation and streamline stable transgenic Marchantia polymorpha line generation.
To enable efficient high-throughput experimentation in plant synthetic biology.

Main Methods:

Developed a freeze-thaw Agrobacterium transformation method adaptable to robotic automation.
Implemented a semi-automated protocol using the Opentrons platform.
Simplified stable transformation and selection of M. polymorpha, including sucrose in selection media.

Main Results:

Achieved efficient Agrobacterium transformation with a freeze-thaw method.
Semi-automated protocol showed comparable efficiency to manual methods.
Streamlined M. polymorpha transformation reduced cost, time, and labor without compromising efficiency.
Sucrose addition enhanced gemmae production, accelerating isogenic plant generation.
Successfully screened hundreds of fluorescent reporters in Marchantia gemmae.

Conclusions:

Optimized protocols facilitate efficient high-throughput plant transformation.
The methods significantly reduce costs and time for generating transgenic plant lines.
These advancements pave the way for automated plant transformation pipelines and broader applications in plant synthetic biology.