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Updated: May 14, 2025
A High Output Method to Isolate Cerebral Pericytes from Mouse
Published on: January 14, 2020
Protocol for extraction and characterization of mouse brain-derived extracellular matrix for neuronal cell culture
Weihao Zhao1, Ziqian Liu1, Zhenghao Li1
1The Sichuan Provincial Key Laboratory for Human Disease Gene Study, The Department of Medical Genetics, Sichuan Academy of Medical Sciences & Sichuan Provincial People's Hospital, University of Electronic Science and Technology of China, Chengdu 610054, China; School of Medicine, University of Electronic Science and Technology of China, Chengdu 610054, China.
Abstract:
Neuronal cell cultures are highly sensitive to their microenvironment, particularly the choice of coating substrate. Here, we present a protocol to isolate extracellular matrix (ECM) from mouse brain tissue, providing a native coating material that closely mimics in vivo conditions. We detail decellularization steps, guidelines for measuring ECM quality, and instructions for co-culturing neuronal cells on the resulting substrate. By promoting improved neuronal survival, growth, and differentiation, this protocol has broad implications for in vitro neurobiological research and downstream applications.
