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Automated Modular High Throughput Exopolysaccharide Screening Platform Coupled with Highly Sensitive Carbohydrate Fingerprint Analysis
Published on: April 11, 2016
Efficient production, structural characterization and bioactivity of an extracellular polysaccharide from Grifola
Dongchao Xie1, Ruixue Zhang1, Jiajun Huang1
1National Key Laboratory for Development and Utilization of Forest Food Resources, Zhejiang A&F University, China.
Abstract:
Endophytic bacteria Burkholderia sp. (GFB) was firstly identified and isolated from Grifola frondosa. An exopolysaccharide (GFB-MP) of GFB strain was obtained following fermentation optimization, resulting in a maximum yield of 11.36 g/L in 5 L fed-batch fermentation. GFB-MP (MW 432.05 kDa) comprised mainly galactose, glucose, and mannose with a ratio of 39.52:14.22:46.26, indicating a mannose-enriched polysaccharide. Methylation and NMR analysis revealed that GFB-MP consisted of the main chain that was repeat units →4)-α-D-Glcp-(1 → bonded →6)-β-D-Galp-1 → repeat units and three O-6-linked branched chains. Antibacterial activity suggested that GFB-MP can effectively inhibit food pathogen bacteria Listeria and Escherichia coli with inhibition ratios of 73.4 % and 81.6 %, respectively. In addition, GFB-MP exhibited remarkable growth-promoting activity on probiotics with >50 % increments of cell growth. This study demonstrates that GFB-MP has the potential for health-beneficial food. Knowledge of endophyte polysaccharides in G. frondosa is important to understand their physiological activities and symbiotic interactions.
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