Surface-Modified Micro-Ring-Resonator Sensors for HMGB1 Detection on a Chip-Scale Platform

Area of Science:

• Biomedical Engineering
• Nanotechnology
• Biosensing

Background:

• High mobility group box 1 (HMGB1) is a key mediator and biomarker in inflammatory diseases, necessitating precise detection methods.
• Silicon on insulator (SOI) integrated waveguide biosensors offer high sensitivity, facile functionalization, and CMOS compatibility for enhanced HMGB1 sensing.

Purpose of the Study:

• To develop a chip-scale, cost-effective micro-ring resonator (MRR) biosensor for quantitative HMGB1 detection.
• To evaluate the sensitivity and accuracy of the functionalized MRR sensor for HMGB1 concentration determination.

Main Methods:

• Fabrication of a micro-ring resonator (MRR) biosensor on a silicon on insulator (SOI) platform.
• Surface functionalization of the MRR using a chemical bonding method with HMGB1 antibodies.
• Quantitative analysis of HMGB1 concentration using the functionalized MRR sensor.

Main Results:

• The HMGB1 sensor achieved a high sensitivity of 1.75 pm/nM.
• A low detection limit of 0.13 μg/mL (4.99 nM) was demonstrated.
• Accurate determination of HMGB1 concentration in mixed solutions with an average error of 5.4%.

Conclusions:

• The developed surface-modified HMGB1 antibody MRR sensor exhibits excellent performance for HMGB1 detection.
• This chip-scale biosensor presents a promising platform for sensitive and cost-effective HMGB1 quantification in inflammatory disease diagnostics.