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Quantum dots encoded superparamagnetic micron-particles based on layer-by-layer assembly for multiplexed nucleic acid

Xiaoshuang Zhao1, Jieying Zhang2, Yifan Chen3

  • 1School of Physics and Optoelectronic Engineering, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, 310024, China; Shanghai Institute of Microsystems and Information Technology, Chinese Academy of Science, Shanghai, 200050, China; University of Chinese Academy of Science, Beijing, 100049, China.

Talanta
|April 14, 2025
PubMed
Summary

This study introduces a new fluorescence bio-coding method using multi-color quantum dots and superparamagnetic particles for multiplexed detection. This approach simplifies bio-analysis and enhances precision medicine diagnostics.

Keywords:
Fluorescence encoded superparamagnetic micron-particlesLayer-by-layer assemblyMultiplexed miRNAs detectionQuantum dot

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Area of Science:

  • Biomedical Engineering
  • Nanotechnology
  • Molecular Diagnostics

Background:

  • Precise medicine demands advanced multiplexed detection technologies.
  • Traditional bio-analysis faces limitations in dye labeling and microscope channels.
  • Microbeads-based fluorescence bio-coding offers potential for enhanced diagnostics.

Purpose of the Study:

  • To develop a novel fluorescence encoding strategy for multiplexed bio-analysis.
  • To create fluorescence encoded superparamagnetic particles (FESPs) for enhanced detection.
  • To simplify bio-analysis processes for biomedical detection and diagnosis.

Main Methods:

  • Utilized multi-color quantum dots (QDs) as fluorescence tags.
  • Employed micron-sized superparamagnetic particles as encoding carriers.
  • Constructed FESPs via metal coordination-based layer-by-layer assembly.
  • Developed FESP probes by binding DNA capture oligonucleotides for miRNA detection.

Main Results:

  • Successfully constructed FESPs with distinct fluorescence colors and intensities.
  • Demonstrated excellent specificity in multiplexed detection of miRNA-21, miRNA-122, and miRNA-130b.
  • Achieved rapid separation of miRNA targets within 5 seconds due to superparamagnetism.

Conclusions:

  • The novel FESP strategy offers a simplified and efficient approach to multiplexed bio-analysis.
  • This technology holds promise for advancing precise medicine and biomedical diagnostics.
  • The developed method overcomes limitations of traditional fluorescence bio-coding techniques.