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Specific mitotic events drive left-right organizer development.

Yan Wu1,2, Yiling Lan1,2, Favour Ononiwu1,2

  • 1Department of Biology, Syracuse University, Syracuse, NY 13244, USA.

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|April 15, 2025
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Summary
This summary is machine-generated.

Cell division timing is critical for Kupffer

Keywords:
Cell patterningCiliaCytokinesisLeft-right organizerLumen formationMicrotubulesMitosisRosette

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Area of Science:

  • Developmental biology
  • Cell biology
  • Zebrafish model systems

Background:

  • Cell proliferation is fundamental for tissue development and organogenesis.
  • The left-right organizer establishes the body's left-right axis, a process critical for organ positioning.
  • Kupffer's vesicle (KV) in zebrafish serves as a model for studying left-right axis determination.

Purpose of the Study:

  • To investigate the role of cell proliferation and mitotic events in the development and function of Kupffer's vesicle (KV).
  • To elucidate the relationship between mitotic timing, KV architecture, and left-right axis determination.

Main Methods:

  • Mapping mitotic events within the zebrafish KV using live imaging.
  • Employing laser ablation techniques to perturb mitotic cells.
  • Analyzing pericentrin-null mutants exhibiting reduced mitotic activity.
  • Utilizing a KV-specific fluorescent microtubule marker to visualize cellular structures.

Main Results:

  • Identified an anteriorly enriched, FGF-dependent mitotic pattern in the KV.
  • Demonstrated that reduced cell division (via laser ablation or pericentrin-null mutants) leads to smaller KV lumens and impaired leftward cardiac jogging.
  • Characterized the KV rosette as a transient structure formed by cytokinetic bridges and microtubule bundles, preceding lumen formation.
  • Showed that mitotic events, spindle rotation, and cell extrusion during KV rounding are linked to rosette formation and are essential for normal KV development.

Conclusions:

  • Cell division is essential for KV development, lumen formation, and proper left-right axis determination.
  • The timing and pattern of mitotic events significantly shape KV architecture, including rosette formation and rounding.
  • Mitotic events play a crucial role in establishing KV function necessary for left-right asymmetry.