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Chiral sensing combined with nuclease activity assay to track Cas9 dynamics in solution: ROA and CPL study

Monika Halat1, Magdalena Klimek-Chodacka1, Agnieszka Domagała2,3

  • 1Department of Plant Biology and Biotechnology, Faculty of Biotechnology and Horticulture, University of Agriculture in Krakow, AL. Mickiewicza 21, 31-120 Krakow, Poland. m.halat@urk.edu.pl.

Chemical Communications (Cambridge, England)
|April 15, 2025
PubMed

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Genome editing technologies allow scientists to modify an organism’s DNA via the addition, removal, or rearrangement of genetic material at specific genomic locations. These types of techniques could potentially be used to cure genetic disorders such as hemophilia and sickle cell anemia. One popular and widely used DNA-editing research tool that could lead to safe and effective cures for genetic disorders is the CRISPR-Cas9 system. CRISPR-Cas9 stands for Clustered Regularly Interspaced Short...