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Updated: May 13, 2025

Quantitative Optical Microscopy: Measurement of Cellular Biophysical Features with a Standard Optical Microscope

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Quantitative Phase Imaging with a Meta-Based Interferometric System.

Cheng Hung Chu¹, Chen-Ming Tsai², Takeshi Yamaguchi³

¹YongLin Institute of Health, National Taiwan University, Taipei 10672, Taiwan.

ACS Applied Materials & Interfaces

|April 15, 2025

Summary

This summary is machine-generated.

A novel meta-based interferometric system provides quantitative phase imaging for transparent specimens. This advanced digital holographic microscopy (DHM) offers enhanced stability and precision for biomedical research and diagnostics.

Keywords:

dielectric metasurface diffractive optics digital holography interferometric microscopy quantitative phase imaging

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Area of Science:

Biomedical Optics
Metasurface Applications
Quantitative Phase Imaging

Background:

Traditional optical phase imaging lacks quantitative phase information.
Off-axis digital holographic microscopy (DHM) is susceptible to environmental noise and requires bulky components.
There is a need for compact, stable, and quantitative phase imaging techniques.

Purpose of the Study:

To develop a meta-based interferometric quantitative phase imaging system.
To utilize a common-path off-axis DHM configuration for improved stability.
To demonstrate the system's capability in retrieving quantitative phase information from biological samples.

Main Methods:

Designed and fabricated a meta-biprism using GaN nanopillars as a compact beam splitter.
Implemented a common-path off-axis digital holographic microscopy (DHM) setup.
Validated the system using standard resolution phase targets and human lung cell lines.

Main Results:

Successfully retrieved quantitative phase information from samples.
Demonstrated enhanced temporal phase stability compared to conventional off-axis DHM.
Showcased the system's effectiveness with biological specimens.

Conclusions:

Metasurfaces can significantly advance quantitative phase imaging capabilities.
The developed system offers a compact, stable, and precise solution for biomedical imaging.
This technology holds promise for improved biomedical imaging and diagnostics.