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Published on: August 16, 2018
Identification of candidate genes in sesquiterpenoid biosynthesis of Atractylodes lancea through combined metabolomic
Chengcai Zhang1, Ye Cao2, Huaibin Lin3
1State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs, National Resource Center for Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, 100700, PR China; Dexing Research and Training Center of Chinese Medical Sciences, Dexing 334220, PR China; Institute for Ecology of Chinese Materia Medica Resources, Deqing, 313200, PR China.
Abstract:
Atractylodes lancea is a type of traditional Chinese medicine whose rhizome is rich in sesquiterpenoids. However, the mechanisms underlying the synthesis and metabolism of sesquiterpenoids in A. lancea remain poorly discovered. In the present research, the organs of A. lancea in different growth and development stages served as test materials. Initially, widely targeted metabolomic and transcriptomic analyses were integrated to elucidate sesquiterpenoid biosynthesis in A. lancea. Up to 177 differentially expressed sesquiterpene metabolites and 80 genes associated with the synthesis pathway of sesquiterpenes were identified. Of these, 13 terpene synthases (TPSs), including AlTPS17, AlTPS20, and AlTPS13, appeared to be directly involved in the synthesis of sesquiterpenes accumulated in the rhizomes. Both AlTPS13 and AlTPS20 used farnesyl diphosphate as a substrate to generate sesquiterpenoids, while AlTPS13 catalyzed the biosynthesis of γ-elemene. This study analyzed the quality-generation pattern of A. lancea under seasonal variations and thereby laid a strong foundation to further elucidate the mechanism of sesquiterpenoid biosynthesis.
