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Related Experiment Videos

Histaminase release from human eosinophils.

R S Zeiger, H R Colten

    Journal of Immunology (Baltimore, Md. : 1950)
    |February 1, 1977
    PubMed
    Summary
    This summary is machine-generated.

    Human eosinophils release histaminase, arylsulfatase, and beta-glucuronidase upon phagocytosis. Eosinophil histaminase release differs from neutrophils, indicating distinct release mechanisms.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Biochemistry

    Background:

    • Eosinophils are key immune cells involved in inflammatory responses.
    • Granulocyte functions, including enzyme release, are critical for host defense and tissue modulation.
    • Understanding specific enzyme release mechanisms is vital for targeting inflammatory diseases.

    Purpose of the Study:

    • To investigate the release of histaminase, arylsulfatase, and beta-glucuronidase from human eosinophils.
    • To compare the mechanisms of enzyme release induced by zymosan and calcium ionophore A23187.
    • To explore differences in histaminase release between eosinophils and neutrophils.

    Main Methods:

    • Human eosinophils and neutrophils were isolated.
    • Cells were stimulated with opsonized zymosan and calcium ionophore A23187.

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  • Enzyme release (histaminase, arylsulfatase, beta-glucuronidase) was measured.
  • The effect of cytochalasin B on enzyme release was assessed.

    • Main Results:

    • Phagocytosis of opsonized zymosan induced a dose-dependent, noncytotoxic release of histaminase, arylsulfatase, and beta-glucuronidase from eosinophils.
    • Calcium ionophore A23187 stimulated eosinophil histaminase release at lower concentrations than arylsulfatase and beta-glucuronidase.
    • Zymosan-induced histaminase release was significantly reduced in eosinophils treated with cytochalasin B, but not in neutrophils.

    Conclusions:

    • Eosinophils release multiple enzymes in response to phagocytic stimuli.
    • Distinct mechanisms govern histaminase release in eosinophils compared to neutrophils.
    • These findings highlight potential differences in granulocyte activation pathways.