Perfluorocarbone (FDA 20) and extracorporeal circuit of isolated extremity perfusion

The applicability of Fluosol DA 20 as a perfusion fluid was investigated in the isolated limb perfusion of 14 mongrel dogs and its performance compared with that of diluted blood. Control of perfusion parameters permitted a direct comparison of the two perfusion liquids. Muscle tissue, blood and perfusate were analyzed for pH, pO2, pCO2, O2 content, lactate, pyruvate, ATP and creatine phosphate. Due to given experimental conditions, the pH values of the perfusion liquid and muscle tissue were in a slightly acidic range. The oxygen uptake of the perfusion liquids and the oxygenation of tissue was satisfactory, the metabolism of the muscle tissue was not disrupted.