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Related Experiment Video

Updated: May 11, 2025

The bm12 Inducible Model of Systemic Lupus Erythematosus SLE in C57BL/6 Mice
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Study on the Correlation Between Double-Stranded DNA and Systemic Lupus Erythematosus.

Qin Qin1,2, Yirui Wang1,2, Sihao Yan1,2

  • 1Department of Dermatology, The First Affiliated Hospital of Anhui Medical University, Hefei, China.

Experimental Dermatology
|April 17, 2025
PubMed
Summary
This summary is machine-generated.

Elevated levels of double-stranded DNA (dsDNA) in systemic lupus erythematosus (SLE) patients show potential for disease assessment. Measuring serum dsDNA may offer a simpler diagnostic and management tool for SLE.

Keywords:
double‐stranded DNAexosomesystemic lupus erythematosus

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Area of Science:

  • Immunology
  • Molecular Biology
  • Rheumatology

Background:

  • Circulating cell-free DNA (cfDNA) is implicated in Systemic Lupus Erythematosus (SLE) pathogenesis.
  • Current cfDNA detection lacks diagnostic consistency for SLE.
  • The role of double-stranded DNA (dsDNA), a cfDNA component, in SLE requires further investigation.

Purpose of the Study:

  • To investigate the correlation between serum dsDNA levels and SLE.
  • To explore the relationship between serum dsDNA and exosome dsDNA in SLE patients.
  • To assess the potential of dsDNA as a biomarker for SLE diagnosis and disease progression.

Main Methods:

  • Serum dsDNA concentration was measured using a fluorescence quantitative method.
  • Propensity score matching (PSM) was employed to compare 148 SLE patients with 444 healthy controls.
  • Blood exosomes were extracted to analyze serum and exosome dsDNA correlation.

Main Results:

  • SLE patients exhibited significantly higher serum dsDNA levels compared to healthy controls.
  • Serum dsDNA levels in SLE patients correlated with season and other clinical indicators.
  • A significant association was found between serum dsDNA and exosome dsDNA, with upregulation of dsDNA receptor genes.

Conclusions:

  • Serum dsDNA shows promise as a rapid and simple tool for assessing SLE disease progression.
  • Further research is needed to elucidate the mechanism of elevated dsDNA in SLE.
  • dsDNA detection could aid physicians and patients in SLE management.