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Updated: May 11, 2025

A Protocol for Functional Assessment of Whole-Protein Saturation Mutagenesis Libraries Utilizing High-Throughput Sequencing
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Structural Analysis and Mutability Landscape-Guided Engineering of ω-Transaminase for Improved Catalytic Performance

Puhong Yi1,2,3, Yue Xu1,2,3, Yuhua Hao1,2,3

  • 1The National and Local Joint Engineering Research Center for Biomanufacturing of Chiral Chemicals, Zhejiang University of Technology, Hangzhou 310014, P. R. China.

Journal of Agricultural and Food Chemistry
|April 17, 2025
PubMed

Keywords:
distal mutationloop flexibilitymutability landscapestructure-guided engineeringω-transaminases

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