Related Experiment Video
Updated: May 1, 2026
Utilization of Capsules for Negative Staining of Viral Samples within Biocontainment
Published on: July 19, 2017
Protocol for mpox virus inactivation in containment level 3 for safe handling of animal samples
Benedicte Mpia Moni1, Arianna Mahely Hurtado-Monzón2, Jill Van Kessel2
1Vaccine and Infectious Disease Organization (VIDO), University of Saskatchewan, Saskatoon, SK S7N 5E3, Canada; Department of Virology, National Institute for Biomedical Research, Kinshasa, DR Congo; Department of Biology, Faculty of Science and Technology, BP 190 Kinshasa XI, University of Kinshasa, Kinshasa, DR Congo; School of Medicine, Catholic University of Congo, Kinshasa, DR Congo.
Abstract:
High biocontainment is required to handle infectious mpox virus (MPXV) specimens, which can hinder research and diagnostic efforts during outbreaks. Here, we present a protocol for inactivating MPXV-contaminated animal samples using commercial lysis buffers. We describe steps for virus propagation and quantification and MPXV inactivation. We then detail procedures for validating the inactivation using a cell-based technique and qPCR. This protocol provides a framework for safely managing MPXV in lower-containment laboratories, facilitating downstream applications and improved outbreak management.
Insights
This study introduces a new protocol to inactivate the mpox virus (MPXV) in animal samples using lysis buffers. This method allows for safer handling in lower-containment labs, aiding research and outbreak response.
Area of Science:
- Virology
- Biotechnology
- Public Health
Background:
- High biocontainment is necessary for handling infectious mpox virus (MPXV) specimens, posing challenges for research and diagnostics during outbreaks.
- MPXV research and diagnostics are often limited by the stringent safety requirements of high biocontainment facilities.
Purpose of the Study:
- To develop and validate a protocol for inactivating MPXV in contaminated animal samples using commercially available lysis buffers.
- To enable safe handling and downstream analysis of MPXV samples in lower-containment laboratory settings.
Main Methods:
- Virus propagation and quantification of MPXV.
- MPXV inactivation using commercial lysis buffers.
- Validation of inactivation efficacy through cell-based assays and quantitative polymerase chain reaction (qPCR).
Main Results:
- A detailed protocol for MPXV inactivation was successfully developed and validated.
- The lysis buffer method effectively inactivated MPXV in contaminated animal samples.
- Validation confirmed the absence of infectious MPXV after treatment.
Conclusions:
- The presented protocol offers a safe and effective method for managing MPXV-contaminated samples in laboratories with lower biocontainment levels.
- This framework facilitates essential downstream applications, such as molecular testing and research, thereby improving MPXV outbreak management strategies.
Related Concept Videos
01:30Cleaning, Sterilization, and Disinfection
Cleaning
The cleaning process usually involves using water with detergents or enzymatic cleaner and removing foreign material from objects and surfaces, including organic material such as body fluids or inorganic material like soil. Cleaning is performed before high-level disinfection and sterilization because foreign materials on the cover of the devices interfere with process...
01:27Chemical Agents for Microbial Control
01:28Biological Methods for Microbial Control